Selected Publications

  1. Owens JL, Beketova E, Liu S, Shen Q, Pawar JS, Asberry AM, Yang J, Deng X, Elzey BD, Ratliff TL, Cheng L, Choo R, Citrin DE, Polascik TJ, Wang B, Huang J, Li C, Wan J, Hu CD. Targeting Protein Arginine Methyltransferase 5 Suppresses Radiation-induced Neuroendocrine Differentiation and Sensitizes Prostate Cancer Cells to Radiation. Mol Cancer Ther., doi: 10.1158/1535-7163 (2022)                                                                                                                       

  2. Beketova, E, Owens, JL, Asberry, A, Hu, CD. PRMT5: A putative oncogene and therapeutic target in prostate cancer. Cancer Gene Ther., doi: 10.1038/s41417-021-00327-3 (2021)                                                                                                           

  3. Beketova, E., Fang, S., Owens, J.L., Liu, S., Chen, X., Zhang, Q., Asberry, A.M., Deng, X., Malola, J., Huang, J., Li, C., Pili, R., Elzey, B.D., Ratliff, T.L., Wan, J., and Hu, C.D. Cancer Res, (2020)                                                                                                                                                                                                                  

  4. Liu, S., Shen, J., Fang, S., Li, K., Liu, J., Yang, L., Hu, C.D., and Wan, J. Genetic spectrum and distinct evolution patterns of SARS-CoV-2. Front Microbiol, (2020)                                                       

  5. Jia, Z., Yue, F., Chen, X., Narayanan, N., Qiu, J., Syed, S.A., Imbalzano, A.N., Deng, M., Yu, P., Hu, C.D. and Kuang, S. Protein arginine methyltransferase PRMT5 regulates fatty acid metabolism and lipid droplet biogenesis in white adipose tissues. Advanced Science, Accepted for publication (2020)                                                                                                         

  6. Li, Y.H., Tong, K.L., Lu, J.L., Lin, J.B., Li, Z.Y., Yang, J., Sang, Y., Ghodbane, A., Liu, N., Gao, X.J., Tam, M.S., Hu, C.D. Zhang, H.T., and Zha, Z.G. PRMT5-TRIM21 interaction regulates the senescence of osteosarcoma cells by targeting the TXNIP/p21 axis. Aging, 12:2507-2529 (2020)                                                                                                                              

  7. Owens, J.L., Beketova, E., Liu, S., Tinsley, S.L., Asberry, A.M., Deng, X., Huang, J., Li, C., Wan, J., Hu, C.D. PRMT5 Cooperates with pICln to Function as a Master Epigenetic Activator of DNA Double-Strand Break Repair Genes. iScience, 23:100750 (2020)  

  8. Doyle, T.B., Muntean, B.S., Ejendal, K.F., Hayes, M.P., Soto-Velasquez, M., Martemyanov, K.A., Dessauer,, C.W., Hu, C.D., Watts,V.J. Identification of Novel Adenylyl Cyclase 5 (AC5) Signaling Networks in D1 and D2 Medium Spiny Neurons using Bimolecular Fluorescence Complementation Screening. Cells, 8:E1468 (2019) 

  9. Vickman, R.E., Yang, J., Lanman, N.A., Cresswell, G.M., Zheng, F., Zhang, C., Doerge, R.W., Crist, S.A., Mesecar, A.D., Hu, C.D., and Ratliff, T.L. Mol Cancer Res, 17:1253-1263 (2019)

  10. Zeng, L., Wang, W.H., Arrington, J., Shao, G., Geahlen, R.L., Hu, C.D. and Tao, W.A. Identification of upstream kinases by fluorescence complementation mass spectrometry. ACS Central Sci, (2017)

  11. Deng, X., Shao, G., Zhang, H.T., Li, C., Zhang, D., Cheng, L., Elzey, B.D., Pili, R., Ratliff, T.L., Huang, J. and Hu, C.D. Protein arginin methyltransferase 5 functions as an epigenetic activator of the androgen receptor to promote prostate cancer cell growth. Oncogene, 36:1223-1231 (2017)

  12. Vickman, R.E., Christ, S.A., Kerian, K., Eberlin, L., Coos, R.G., Burcham, G.N., Buhman, K.K., Hu, C.D., Mesecar, A.D., Cheng, L., Ratliff, T.L. Cholesterol sulfonation enzyme, SULT2B1b, modulates AR and cell growth proerties in prostate cancer. Mol Cancer Res, 14:776-789 (2016)

  13. Zhang, H.T., Zheng, L.F., He, Q.Y., Tao, W.A., Zha, Z.G., and Hu, C.D. The E3 ubiquitin ligase CHIP mediates ubiquitination and proteasomal degradation of PRMT5. Biochim Biophys Acta 1863:335-346 (2016)

  14. Pratt, E.P., Owens, J.L., Hockerman, G.H., and Hu, C.D. Bimolecular fluorescence complementation (BiFC) analysis of protein-protein interactions and assessment of subcellular localization in live cells. Methods Mol Biol, 1474:153-170 (2016)

  15. Xu, D., Zhan, Y., Qi, Y., Cao, B., Bai, S., Xu, W., Gambhir, S.S., Lee, P., Sartor, O., Flemington, E.K., Zhang, H., Hu, C.D., and Dong, Y. Androgen receptor splice variants dimerize to transactivate target genes. Cancer Res 75:3663-3671 (2015)

  16. Hu, C.D., Choo, R., and Huang, J. Neuroendocrine differentiation in prostate cancer: a mechanism of radioresistance and treatment failure. Front Oncol 5:90. doi: 10.3389/fonc.2015.00090

  17. Suarez, C., Deng, X., and Hu, C.D. Targeting CREB inhibits radiation-induced neuroendocrine differentiation and increases radiation-induced cell death in prostate cancer cells. Am J Cancer Res 4:850-861 (2014)

  18. Zhang, H.T., Zhang, D., Zha, Z.G., and Hu, C.D. Transcriptional regulation of PRMT5 by NF-Y is required for cell growth and negatively regulated by the PKC/c-Fos signaling in prostate cancer. Biochim Biophys Acta 1839:1330-1340 (2014)

  19. Hsu, C., and Hu, C.D. Transcriptional activity of c-Jun is critical for the suppresson of AR function. Mol Cell Endocrinol 372:12-22(2013)

  20. Ejendal, K.F.K., Conley, J.M., Hu, C.D. and Watts, V.J. Bimolecular fluorescence complementation analysis of G protein-coupled receptor dimerization in living cells. Methods Enzymol 521:259-270 (2013)

  21. Kodama, Y. and Hu, C.D. Bimolecular fluorescence complementation (BiFC): How to calculate signal-to-noise ratio. Methods Cell Biol 113:107-121 (2013)

  22. Kodama, Y. and Hu, C.D. Bimolecular fluorescence complementation (BiFC): A 5-year update and future perspectives. Biotechniques, 53:285-298 (2012)

  23. Young MM, Takahashi Y, Khan O, Park S, Hori T, Yun J, Sharma AK, Amin S, Hu CD, Zhang J, Kester M, Wang HG. Autophagosomal membrane serves as platform for intracellular death-inducing signaling complex (iDISC)-mediated caspase-8 activation and apoptosis. J. Biol. Chem. 287:12455-12688 (2012)

  24. Hsu, C. and Hu, C.D. Critical role of an N-terminal end nuclear export signal in regulation of ATF2 subcellular localization and transcriptional activity. J. Biol. Chem. 287:8621-8632 (2012)

  25. Deng, X., Elzey, B.D., Poulson, J.M., Morrison, W.B., Ko, S.C., Hahn, N.M., Ratliff, T.L., and Hu, C.D. Ionizing radiation induces neuroendocrine differentiation in vitro, in vivo and in human prostate cancer patients. Am. J. Cancer Res. 1:834-844 (2011)

  26. Xing, J., Wang, S., Lin, F., Pan, W., Hu, C.D., and Zheng, C. A comprehensive characterization of interaction complexes of Herpes Simplex Virus type I ICP22, UL3, UL4 and UL20.5. J. Virol. 85:1881-1886 (2011) 

  27. Kodama, Y. and Hu, C.D.. An improved bimolecular fluorescence complementation assay with a high signal-to-noise ratio. Biotechniques, 49:793-805 (2010)

  28. Le, T.T, Duren, H.M., Slipchenko, M.N., Hu, C.D.*, and Cheng, J.X. Label-free quantitative analysis of lipid metabolism in living Caenorhabditis elegans. J. Lipid  Res. 51:672-677 (2010)  *Co-Corresponding Author  

  29. Hiatt, S.M., Duren, H.M. Shyu, Y., Ellis, R.E., Hisamoto, N., Matsumoto, K., Kariya, K., Kerppola, T.K., and Hu, C.D.. C. elegans FOS-1 and JUN-1 regulate plc-1 expression to control ovulation. Mol. Biol. Cell 20:3888-3895 (2009)

  30. Yuan, Z., Gong, S., Song, B., Mei, Y., Hu, C., Li, D., Thiel, G., Hu, C.D., and Li, M. Opposing role for ATF2 and c-Fos in c-Jun-mediated apoptosis induced by potassium deprivation in cerebellar granule neurons. Mol. Cell. Biol. 29:2431-2442 (2009)

  31. Deng, X., Liu, H., Huang, J., Cheng, L., Keller, E.T., Parsons, S.J., and Hu, C.D. Ionizing radiation induces prostate cancer neuroendocrine differentiation through interplay of CREB and ATF2: Implications for disease progression. Cancer Res. 68:9663-9670 (2008)

  32. Shyu, Y. and Hu, C.D. Fluorescence complementation: an emerging tool for biological research. Trends Biotechnol. 26:622-630 (2008)

  33. Shyu, Y., Suarez, C., and Hu, C.D. Visualization of ternary complexes in living cells by using a BiFC-based FRET analysis. Nat. Protoc. 3:1693-1702 (2008)

  34. Shyu, Y., Fox, SM., Duren, HM., Ellis, R.E., Kerppola, T.K. and Hu, C.D. Visualization of protein interaction in living Caenorhabditis elegans using bimolecular fluorescence complementation (BiFC) analysis. Nat. Protoc., 4:588-596 (2008)

  35. Shyu, Y., Suarez, C., and Hu, C.D. Visualization of AP-1-NF-κB ternary complexes in living cells by using a BiFC-based FRET. Proc. Natl. Acad. Sci. U.S.A., 105:151-156 (2008)

  36. Liu, H., Deng, X., Shyu, Y., Li, J.J., Taparowsky, EJ., and Hu, C.D. Mutual regulation of c-Jun and ATF2 by transcriptional activation and subcellular localization. The EMBO J., 25:1058-1069 (2006)

  37. Hu, C.-D. and Kerppola, T. Simultaneous visualization of interactions between multiple proteins in living cells using multicolor bimolecular fluorescence complementation analysis. Nat. Biotechnol. 21, 539-545 (2003)

  38. Hu, C.-D. Chinenov, Y., and Kerppola, T Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol. Cell. 9, 789-798 (2002).